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Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Journal of Veterinary Science ; : 307-316, 2017.
Article Dans Anglais | WPRIM | ID: wpr-57417
ABSTRACT
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéine A staphylococcique / Suidae / Test ELISA / Chromatographie d'affinité / Sensibilité et spécificité / Collodion / Colloïdes / Or colloïdal / Virus du syndrome respiratoire et reproducteur porcin / Syndrome dysgénésique et respiratoire porcin Type d'étude: Etude diagnostique langue: Anglais Texte intégral: Journal of Veterinary Science Année: 2017 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéine A staphylococcique / Suidae / Test ELISA / Chromatographie d'affinité / Sensibilité et spécificité / Collodion / Colloïdes / Or colloïdal / Virus du syndrome respiratoire et reproducteur porcin / Syndrome dysgénésique et respiratoire porcin Type d'étude: Etude diagnostique langue: Anglais Texte intégral: Journal of Veterinary Science Année: 2017 Type: Article