Effect of Liuwei Dihuang Pills Prepared by different Kinds of Techniques on Hemolysin Content in Mice / 中药新药与临床药理

ABSTRACT

Objective To compare the pharmacodynamic actions of Liuwei Dihuang Pills(LDP)prepared by four kinds of techniques on hemolysin contents in mice.Methods Sensitized by SRBC,immuno-inhibited mice models were induced by dexamethasone and cyclophosphane respectively.The hemolysin contents in the mice fed with LDP prepared by four kinds of techniques were observed to compare the pharmacodynamic actions.Results In experiment 1 that immuno-inhibited mice models were induced by cyclophosphane,the half hemolysin concentration(HC50)was low in the model group and LDP groups,especially in the LDP groups of techniqueⅡ,Ⅲ and Ⅳ.In experiment 2 that immuno-inhibited mice models were induced by dexamethasone,the difference of HC50 was insignificant between the blank control group,LDP groups of techniqueⅠand concentrated LDP group,the difference also being not significant in LDP groups of techniqueⅠat different dosages.Conclusion The dose of LDP can be reduced according to the experiment,however,the process technique of LDP needs to be improved.For this prescription is indicated for the treatment of yin-deficiency syndrome,dexamethasone can be used as the proposed drug for the establishment of the immuno-inhibited models.To confirm the reasonability of formula revolution,more parameters should be added to observe the pharmacodynamic actions.