Determination of Notoginsenoside R_1 and Ginsenoside Rg_1, Rb_1 in Naodesheng Capsules by HPLC Gradient Elution Method / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-574878
ABSTRACT
Objective To establish a method of HPLC gradient elution method determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules. Methods Kromasil C18 column was used with a mobile phase of acetonitrile-water gradient elution, column temperature at 25 ℃. Results Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1 showed good linear relationship at the range of 0.495~1.782 ?g (r =0.9999), 2.65~9.54 ?g (r =0.9995), 1.85~6.66 ?g (r =0.9999), respectively. The average recoveryNotoginsenoside R1 was 100.60% (RSD=1.99%), Ginsenoside Rb1 was 100.86% (RSD=2.19%) and Ginsenoside Rg1 was 98.83% (RSD=1.95%), respectively. Conclusion The methods is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules.
Texte intégral:
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Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Information on Traditional Chinese Medicine
Année:
2006
Type:
Article
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