HPLC Fingerprint of Danggui Buxue Decoction(Ⅰ) / 中药新药与临床药理

ABSTRACT

Object To establish the fingerprint of isoflavones and ferulic acid of Danggui Buxue decoction.Methods HPLC with Hypersil ODS column was adopted,the methanol-0.2 % acetic acid glacial(gradient elution) as mobile phase with a flow rate of 1mL/min and detecting wavelength at 254 nm.Results There were 10 main peaks in Danggui Buxie decoction,9 of them came from Radix astragali and 3 came from Radix angelicae sinensis.Conclusion This fingerprint can be used as a reference for the stability of the isoflavones and ferulic acid in Danggui Buxue decoction.