Establishment of Animal Model of Coronary Heart Disease with the Blending of Phlegm and Blood Stasis / 广州中医药大学学报

ABSTRACT

【Objective】To explore a method for the establishment of animal model of coronary heart disease(CHD) with the blending of phlegm and blood stasis.【Methods】Methods of TCM-WM combination(traditional Chinese medicine integrated with western medicine) and disease-syndrome combination were used to establish a model.The model was evaluated by the changes of symptoms,signs and laboratory indexes and by the effect of blood-stasis-removing and phlegm-resolving herbs on the model.Sixty Wistar rats were randomized into normal group,model group,low-dose herbal medicine group and high-dose herbal medicine group.The normal group was fed with routine forage and normal saline,and the other three groups with high-fat forage and fat milk(10mL?kg~(-1)?d~(-1)).Low-and high-dose herbal medicine groups were given Xuefu Zhuyu Decoction combined with Gualou Xiebai Banxia Decoction in the dosages of 12 and 24 10g?kg~(-1)?d~(-1)respectively by gastric gavage,and the model group given the same volume of normal saline for 7 consecutive weeks.Coronary artery ligation was performed in the 35~(th) day.The changes of electrocardiogram(ECG) were observed before and after the modeling,and blood lipid,myocardial enzymes and hemorrheological parameters were determined.The heart weight was examined to calculate heart index.【Results】ECG in the model group indicated severe myocardial ischemia,which may be relieved by herbal medicine.Serum contents of cholesteril,tryglyceride and creatine kinase(CK),whole blood viscosity,hematocrit and heart index were obviously increased in the model group,while the above indexes decreased to different degrees in herbal medicine groups (P