Determination of 2 caffeic acid derivatives in Echinacea extracts by RP-HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
Dans Chinois
| WPRIM
| ID: wpr-575895
ABSTRACT
AIM:
To estahlish a RP-HPLC method of determining two caffeic acid derivatives in Echinacea extract and to determine 5 Echinacea purpurea root extracts.METHODS:
RP-HPLC was applied to determine 2 caffeic acid derivatives in E.purpurea extractcaftaric acid and chicoric acid.HPLC conditions were as follows Agilent ZORBAX StableBond C_(18) column(5 ?m,4.6 mm?250 mm) was used,with A(acetonitrile) —B(0.1% H_3PO_4) gradient elution as a mobile phase.The flow rate was 1.2 mL/min.The detection wavelenth was set at 330 nm.RESULTS:
2 caffeic acid derivatives were separated well.The linear range for caftaric acid and chicoric acid were 0.064-0.416 ?g and 0.1-0.7 ?g,respectively.Its average recoveries were 99.37% with RSD of(1.50%) and 100.44% with RSD of 1.71%,respectively.CONCLUSION:
The method is simple,accurate,strong specificity and can be used to control the quality of E.purpurea extract and new medicine development.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Traditional Patent Medicine
Année:
1992
Type:
Article
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