Effects of peroxisome proliferator-actived receptors-? agonists on tumor necrosis factor-? expression in rats with acute experimental autoimmune encephalomyelitis / 重庆医科大学学报

ABSTRACT

Objective:To explore the effects of peroxisome proliferator-actived receptors-?(PPAR-?)agonists rosiglitazone and non-steroidal anti-inflammatory drugs(NSAIDs) ibuprofen on tumor necrosis factor-?(TNF-?)mRNA expression in the brain of rats with acute experimental autoimmune encephalomyelitis(EAE).Methods:EAE models on rat were established by inoculating the homogenate containing spinal cord of guinea pig and complete Freund's adjuvant,and then treated by rosiglitazone and ibuprofen respectively.The improvement of clinical manifestations was assessed to determine the curative effect of the two medicines and TNF-? mRNA expression level was detected by RT-PCR.Results:Compared with EAE group,the groups with rosiglitazone and ibuprofen showed improvement in clinical manifestations ,and the expression of TNF-? mRNA reduced markedly(P0.05).Conclusions:PPAR-? agonists rosiglitazone and NSAIDs ibuprofen could obviusely improve the clinical manifestations of EAE rats,and decrease the level of the expression of TNF-? mRNA,which suggested PPAR-? agonists may have the anti-inflammation neural protective function on EAE or multiple sclerosis(MS),and the anti-inflammation of ibuprofen may be related to the activation of PPAR-?.