Construction of mouse peritonitis models of Enterococcus / 重庆医科大学学报

ABSTRACT

Objective:To construct mouse peritonitis models of Enterococcus for the study of the putative virulence factor of Enterococcus.Methods:We used the standard strain OG1RF and sprE gene deficient mutant of Enterococcus faecalis to construct mouse peritonitis models.And the index of peritonitis was detected to measure the virulence decrease of sprE mutant. Results:It was showed that 50%lethal dose(LD50)of sprE mutant was 7 times than that of standard strain OG1RF,and its survival rate was also prominently that of OG1RF in the mouse peritonitis model;while the change of blood leukocytes of sprE mutant group was lower than that of standard stain at 6 hour and 12 hour;So were the TNF-? level in peritoneal fluid and active TNF-? in peritonitis at 6 hour.Conclusion:The peritonitis models of Enterococcus was constructed successfully,which would be used to study other putative virulence factor of Enterococcus face-ali.