Method Research of Measuring Soluble Protein Contents of Animal Material Rough Extraction Using Coomassie Brilliant Blue / 中国中医药信息杂志

ABSTRACT

Objective To study the stability of the Bradford method for determination of soluble protein in animal materials. Method The absorption of CBB G-250 combined with proteins was determined by the UV-Spectrophotometry method with the detection wavelength at 595 nm,and the dried bufo was selected as sample,BSA as control. Results The method showed a good linear relationship between absorption and protein content (r =0.996). The sample was stable with in 10 min (RSD=5.0%). Conclusion This method was reliable,simple and rapid.