The effects of FuYuan capsule on interleukin beta 1 and transforming growth factor beta 2 in articular cartilage of experimental osteoarthritis model / 重庆医科大学学报

ABSTRACT

Objective:To study the effects of FuYuan capsule on articular cartilage degradation and expression of interleukin beta 1 and transforming growth factor beta 2 in osteoarthritis(OA).Methods:Fifty-four healthy New Zealand rabbits received unilateral anterior cruciate ligament transaction(ACTL)to establish model of OA.One week after operation they were randomly divided into 4 groups,named as model control group and FuYuan capsule low,moderate and high dose groups with 12 rabbits in each group.The other 6 rabbits divided into normal control and model control group were given physiological saline.Treatment groups were perfused with FuYuan capsule(727mg/kg、1453mg/kg、4795mg/kg)once a day for 4,8 and 12 weeks.Samples of joint were collected at the 4th,8th,and 12th week after managements for general,light microscopic and electron microcope observation,and the distribution of IL-1?and TGF?2 of cartilage was documented by immunohistochemistry.Cartilage of medial femoral condyles and tibial plateau at the margin of lesion nearby were obtained and mRNA expression of TGF?2 was detected using reverse transcription-polymerase chain reaction(RT-PCR).Results:Cartilage degeneration in FuYuan capsule group was significantly slighter than that in model control group.The levels of IL-1? in treatment groups were lower significantly than those in model control group(P