Effects of Fluid Shear Stress on IL-8 mRNA Expression in EA.Hy926 Cells / 航天医学与医学工程

ABSTRACT

Objective To investigate the effects of fluid shear stress on interleukin-8(IL-8)mRNA expression in human endothelial cell line-EA.Hy926 cells.Methods Weibel-Palade body and factor Ⅷ related antigen were detected to identify cultured EA.Hy926 cells.Quantitative reversal transcription-polymerase chain reaction was also used to assay IL-8 mRNA expression.Results It was found that the growth feature of EA.Hy926 cells in vitro culture was similar with that of human umbilical vein endothelial cells(HUVECs).Meanwhile,it also had the typical features of endothelial cells,i.e.Weibel-Palade body in plasma and express factor Ⅷ related antigen.IL-8 mRNA expression of endothelial cells exposed to low shear stress(0.420 Pa)increased at 1 h and reached its peak value at 2 h,then gradually decreased at 3 h and kept the descending trend throughout the remained time course of the study,as compared with that in cells not treated with shear stress.Also after exposed to shear stress of different levels(0.182,0.420,1.000,1.640 Pa)for 2 h,in which IL-8 mRNA expression of EA.Hy926 cells decreased with the increase of the intensity of the shear stress.Conclusion The results suggest that fluid stress can induce the expression of IL-8 mRNA in EA.Hy926 cells.EA.Hy926 cells might be used as a cell source in the field of biorheological research of endothelial cells.