Effects of Psoralen on ADR Multidrug Resistance and Ca~(2+)Concentration in MCF-7/ADR Cells / 航天医学与医学工程
Space Medicine & Medical Engineering
;
(6)2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-577251
ABSTRACT
Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. ]Results Psoralen from 1 to 20 ?mol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Space Medicine & Medical Engineering
Année:
2006
Type:
Article
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