Study of cell-mediated immune response induced by HPV DNA vaccine in combination with IL-15 expression plasmid / 中国艾滋病性病
Chinese Journal of AIDS & STD
;
(6)2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-577309
ABSTRACT
Objective To investigate the effects of IL-15 eukaryotic expression plasmid on the Ag-specific immune responses induced by HPV 16 E7 vaccine in mice.Methods An eukaryotic expression plasmid encoding IL-15 gene coding region was constructed and named as pcDNA3.1IL-15.Female BALB/c mice were injected intramuscularly with pcDNA3.1-IL-15 and HPV16 E7 gene vaccine.After the immunization,the concentration of serum IFN-? were tested.Single-cell suspensions of splenocytes were prepared from mice and were re-stimulated with HPV16 E7 protein.Then,the T cell proliferation assay was measured by the MTT method.Results The concentration of serum IFN-? in mice after co-injection with pcDNA3.1-IL-15 and pcDNA3.1-E7 was raised to 414.1pg/ml,which was significantly higher than that of mice co-injected with pcDNA3.1 and pcDNA3.1-E7 or mice injected with pcDNA3.1-E7 only(P
Texte intégral:
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Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of AIDS & STD
Année:
2007
Type:
Article
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