Evaluation on cytotoxicity of propolis and calcium hydroxide / 重庆医科大学学报

ABSTRACT

Objective:To evaluate the cytotoxicity of propolis and calcium hydroxide for human gingival fibroblast.Methods:Human gingival fibroblast(HGF)was incubated in culture solution containing propolis or calcium hydroxide at different concentrations.The relative growth rates(RGR)were examined by method of MTT assay and cytotoxicity was classified by the five-class method.Then the results were statistically analysed.Results:The cytotoxicities of both 0.1% to 0.6% propolis and calcium hydroxide solutions at different concentration were all graded as first or second class.There was no statistical difference between them.Conclusion:Propolis and calcium hydroxide have weak cytotoxicity for HGF and they are all safe as local application for oral diseases.