Effect of Shenkang Pills on Proliferation,Extracellular Matrix Secretion and Cell Cycle of In-vitro Cultured Rat Mesangial Cells / 广州中医药大学学报

ABSTRACT

【Objective】To observe the effect of Shenkang Pills(SP) on proliferation,extracellular matrix(ECM) secretion and cell cycle of in-vitro cultured rat mesangial cells(MC).【Methods】Sixteen Wistar male rats were randomized into 4 groups: normal serum group,Capoten(5 mg/kg) group,high-and low-dose SP(2.4 and 1.2 g/kg respectively) groups.Except the normal serum group,the rats in other groups received corresponding drugs according to the experimental design,and serum containing drug was isolated and prepared after treatment for 7 days.MC cultured with high glucose(30 mmol/L) in vitro were divided into 5 groups: A(cultured with high glucose 30 mmol/L),B(cultured with 5% normal serum),C(cultured with 5% serum containing Capoten),D and E(cultured with 5% serum containing high-and low-dose SP respectively).Another model group F(cultured with low glucose 10 mmol/L) was also established.After culturing for 72 hours,methyl thiazolyl tetrazolium(MTT) assay was used for the detection of MC proliferation,enzyme-linked immunosorbent assay(ELISA) for fibronectin(FN) content in the culture supernatant and flow cytometer for cell cycle.【Results】The proliferation of MC and FN content in supernatant were increased,and abnormal cell cycle was found in high-glucose group A.SP inhibited MC proliferation,increased the percentage of MC at G0/G1 phase,and decreased the percentage of MC at S phase and G2/M phase.【Conclusion】SP has an inhibition on MC proliferation and ECM secretion induced by high glucose,and its mechanism is probably related with the regulation of abnormal MC cell cycle.