Determination of quercetin, kaempferol,isorhamnetin and flavonoids in Compound Ginkgo Granules by HPLC / 中成药

ABSTRACT

AIM: To establish a method for determining total flavonoids in Compound Ginkgo Granules so as to control the quality. METHODS: HPLC conditions YWG-C_ 18 column, mobile phase V(MeOH)∶V(1%HAC)=50∶50, flow rate 1.0 mL/min, detection?=360 nm, column temperature 25 ℃. RESULTS: The calibration curve of quercetin、 kaempferol and isorhamnetin was linear in range of 0.057 2 -0.286 0 ?g, 0.074 0 -0.370 0 ?g, 0.029 4 -0.147 0 ?g, respectively. The average recovery was 98.39% , and RSD was 1.25 (n=6). CONCLUSION: This method is rapid, accurate and reproducible and could be used for quality control of Compound Ginkgo Granules.