Determination of Rutin in Herba Cirsii by HPLC / 中国中医药信息杂志

ABSTRACT

Objective HPLC was used to establish a method for determination of rutin in herba crisii. Methods Lichrospher C18 column(4.6 mm?200 mm,5?m)was used with methoanol-acetic acid(35∶65) as mobile phase,flow rate being 1.0 mL/min,column temperature at 30℃,detection wavelength at 254 nm.Results The content of rutin in herba crisii was 0.5808 mg/g.Conclusions The method is proved to be simple,rapid,and accurate.It can be applied to content determination of rutin in herba crisii.