Expression of HOXa-10 gene in endometrium mice during early pregnancy / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of HOXa-10 gene in the mouse uterus during embryo implantation. Method: real-time fluorescence quantitative PCR (FQ-PCR) and immunohistochemistry were used to detect the HOXa-10 gene and protein of the mouse of un-pregnant pregnant d1,d2,d3,d4,d5 and d7. Result:The HOXa-10 mRNA/?-actin mRNA expression in pregnant group is higher than that of non-pregnant grnup, and the results showed gradual hoist trend as days past, the HOXa-10 mRNA expression reached the maximum at pregnant d4. Immunohistochemical analysis showed the same results as FQ-PCR. Conclusion:HOXa-10 is persistently expressed in mouse endometrium during the early pregnancy and maybe participates in the regulation process of mouse blastodyst implantation.