Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5? / 重庆医科大学学报

ABSTRACT

Objective:To clone and express colonization factor antigen Ⅰ(CFA/Ⅰ) of enterotoxigenic Escherichia coli (ETEC) in E.coli DH5?. Methods:CFA/Ⅰ gene was cloned by PCR from H10407 and recombined to a expression vector pBV220.This vector was designated pBV220- CFA/Ⅰand transformed into E.coli DH5?. The product was tested by SDS-PAGE and immunogenesis test. Results:The CFA/Ⅰ gene could be expressed in E.coli and contained strong immunogenesis by immune test. Conclusion:This study paved the way for using ETEC CFA/Ⅰ recombinant as a vaccine candidate in future.