Pharmacokinetics of effective constituent in Jinkui Shenqi Pill / 中成药

ABSTRACT

AIM:To investigate pharmacokinetics of cinnamon acid and paeonol in Jinkui Shenqi Pill(Radix Rehmanniae Praeparata,Cortex Cinnamomi,Radix Aconiti Lateralis praeparata,Rhizoma Alismatis,etc.)in rabbit.METHODS:RP-HPLC was used to examine blood concentration of cinnamon acid and paeonol in rabbit which was administred Jinkui Shenqi Pill.Chromatographic condition consisted of Symmetry C_ 18(5?m,3.9 mm ?150 mm)chromato graphic column,mobile phase(methanol-1%glacial acetic acid water-solution(45∶55)),flow rate(0.8 mL/min),column temperature(35 ℃),detection wavelength of cinnamon acid(285 nm),detection wave length of paeonol(274 nm).The serum pharmacokinetic parameters were calculated with 3p87 program.RESULTS:Linear range of cinnamon acid ranged from 0.06 ?g/mL to 15 ?g/mL(r=0.999 7),the lowest detectability was 0.054 ?g/mL.Pharmacokinetic process of paeonol in rabbit could be fitted to two-compartment model;Linear range of paeonol ranged from 0.2 ?g/mL to 10 ?g/mL(r=0.999 9),the lowest detectability was 0.02 ?g/mL.Pharmacokinetic process of cinnamon acid in rabbit could be fitted to one-compartment model.CONCLUSION:HPLC detection of serum concentration of cinnamon acid and paeonol is simple,rapid,highly accurate and sensitive.Pharmacokinetic parameters can reveal pharmacokinetics of cinnamon acid and paeonol of Jinkui Shenqi Pill in rabbit.