Tissue culture from cotyledon and hypocotyl explants of Chrysanthemum morifolium / 中草药

ABSTRACT

Objective To seek the optimization hormone combination for the callus induction and differentiation from cotyledon and hypocotyl explants of Chrysanthemum morifolium through tissue culture. Methods Cotyledon and hypocotyl explants were cultured on various medium with different hormone combinations. Results Callus could be inducted on every media designed in this experiment,but the effects to the induction and differentiation culture of buds were different.The results showed that the medium MS+IAA 0.3 mg/L+6-BA 12 mg/L was suitable to the induction of callus from cotyledon and hypocotyl.The optimal hormone combination was MS+IAA 0.3 mg/L+6-BA 4 mg/L for the initiation of adventitious bud of cotyledon.The shoot regeneration percentage reached 67.5%.The optimal medium for the initiation of adventitious bud of hypocotyl was MS+KT 2 mg/L+NAA 0.2 mg/L.The shoot regeneration percentage reached 62.5%.Rooting was induced on 1/2 MS+NAA 0.1 mg/L,and the root inducting ratio reached 100% in 7 d. Conclusion A rapid plantlet regeneration system for C. morifolium is established from cotyledon and hypocotyl explants.Bud induction frequency is higher and the shoots in vitro grow vigorously.