The expression of stem cell pdx-1 in different purity pancreatic islets before and after transdifferentiated culture / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)1987.
Article
de Zh
| WPRIM
| ID: wpr-579167
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To obtain the stem cells in different pancreatic islets purity utilizing free-serum culture medium and investigate the PDX-1 mRNA expression distinction before and after transdifferentiation culture.Methods:Pancreatic islets of male SD rats(islet donors)were isolated by injection of collagenase into pancreatic ducts,and maintained in serum Ham's F-10 for 36h.Then the cells were kept in free-serum Ham's F-10 for 14 days.The last stage was to shift culture media from free-serum Ham's F-10 to free-serum DMEM/F12 that contained KGF,BSA,glucose and ITS.On the 28d,Dithizon was added into the culture flask for observation of 3D cell.The PDX-1 positive cells was investigated by immunocytochemistry.Results:After transdifferentiation culture,some 3D cells were red.The pdx-1 was remarkable in cell nucleus.Conclusion:After transdifferentiation culture,some pancreatic stem cell display the character of islet cell.The daughter cells of pancreatic stem cells have character of stem cells after transdifferentiation.KGF may have effect on promoting the expression of PDX-1.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Chongqing Medical University
Année:
1987
Type:
Article