Expression and postmortem stability of HSP70 in hippocampus of hemorrhagic shock rat / 重庆医科大学学报

ABSTRACT

Objective:To investigate the expression and postmortem stability of HSP70 in hippocampus of hemorrhagic shock rat.Methods:105 healthy SD rats were randomly divided into 5 groupsnormal group,sham operation group,hemorrhagic shock group,traumatic control group and postmortem stability group.In hemorrhagic shock group and postmortem stability group,rats were submitted by controlled bleeding,with the mean arterial pressure kept around 40mmHg.And in traumatic group,brain contusion was induced by piston impact.After that,the regulation of HSP70 expression in hippocampus of each group was detected by immunohistochemistry technique,and evaluated with image analysis technique and statistic system.Results:One hour after hemorrhagic shock,HSP70 expression in hippocampus was significantly higher in each shock subgroups compared with that in normal group and sham group(P0.05).Conclusion:HSP70 is specifically expressed in hippocampus of hemorrhagic shock rat,and showed nice postmortem stability under the environment of -20℃.