Comparison of efficacy of oral,topical and oral & topical itraconazole treatment for experimental Aspergillus keratitis / 重庆医科大学学报

ABSTRACT

Objective:To investigate the therapeutic role of topical, oral and combine of itraconazole treatment using a rabbit model of Aspergillus fumigatus keratitis. Methods: The epithelium was removed from the central cornea and the inoculum of Aspergillus fumigatus blastoconidia was placed on the corneal surface and covered with a contact lens. Forty-eight hours later the 32 rabbits were randomly divided into four groups. In topical group, rabbits were treated with topical itraconazole(1%,eight times daily); in oral group, rabbits received oral itraconazole (40 mg/kg,bid);in combine of group, rabbits were treated between topical itraconazole(1%,eight times daily) and oral itraconazole [40 mg/(kg?d)]. Untreated controls were also set. The eyes were examined and photographed with a slit-lamp 1,3,6, 10,15,20 and 25 days after inoculation, and an observer graded the corneas in a masked fashion. Corneal scraping, cultures were taken on days 3,6,15 and 25 for fungus growth. Results: At the beginning of treatment, with in 1 to 3 days, the slit-lamp scores did not differ among four groups. However, after days 6 the three treated groups had statistically significant lower scores compared to the untreated controls (P