The test of fas ligand gene transfection on inducing renal transplant immunotolerance in rats / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of Fas ligand(FasL) gene transfection to renal allograft in rats. Methods: Firstly Establish renal transplantation model of rat. Secondly FasL recombinant adenovirus vector was contruccted and transduced into rat renal allografts by renal artery perfusion.Immunohistochemistry were used to detect the expression of exogenous FasI gene. Electronic microscopy was used to observe the changes in the ultrastructure.At the same time,mean survival of animals and the level of serum creatinine were observed.Results:Through animal experiment,we found the mean survival time was 31.3 days in Ad-FasL treated group, whereas the the mean survival time of without treatment group was 9.1 days. The mean survival time increased 22 days in Ad-FasL treated group.There has significant difference. Conclusions:Adenoviral vector can successfully transfection rat kidneys with the FasL cDNA. FasL gene transfection can protect rat renal allografts from immunologic attack and prolong the allograft surviva1.