Effects of Fuyuan capsule on expression of IL-1?,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits / 重庆医科大学学报

ABSTRACT

Objective:To observe the effects of fuyuan capsule on change of morphology of synovium,expression of IL-1?、MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits,and investigate the mechanism about the reparation and treatment of fuyuan capsule.Methods:Sixty New Zealand rabbits were divided into five groups randomlynormol group,model contrast group,low dose group, middle dose group and high dose group of fuyuan capsule.OA model was induced in rabbits by improved Hulth'S method.Three Chinese herb groups were treated with fuyuan capsule(0.727,1.453,4.795 g(/kg?d)),continuously for 4,8 weeks and 12 weeks,model contrast group was treated with 0.9%NS,ig,at the same time.Synovium sections were analyzed by immunohistochemistry for the IL-1?,MMP-13 and iNOS,observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic.After the experiment,the differences of these indexes between Three Chinese herb groups and model contrast group were compared.Results:There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P