Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques / 重庆医科大学学报

ABSTRACT

Objective:To establish a two-dimensional polyacrylamide gel electrophoresi(s2-DE)technique forplasma/serumproteomics. Methods:Plasmas from 8 healthy volunteers and 10 with hepatocellular carcinoma(HCC),and sera from 8 HCC were collected.After six proteins of the highest abundance in plasma/sera were depleted by the Multiple Affinity Removal System(MARS)Technologies from Agilent,plasma/sera were subjected to 2-DE.After comparing,analyzing and identifying by the software PD Quest(7.4,0),the enzyme-digested products of differential expression proteins were analyzed by MALDI-TOF-MS.The Peptide mass fingerprinting(PMF) data were obtained and used to identify proteins through NCBInr data bank.Results:In comparison with the healthy volunteers,20 differential proteins were identified in the plasmas of the HCC patients,among which 18 protein expressions were up-regulated and 2 down-regulated.In the sera of the cancer patients,23 differential proteins were identified,among which 17 protein expressions were up-regulated and 6 down-regulated.Tumor necrosis factor-inducible protein TSG-14 and FADD protein were both identified in the plasmas and sera of the cancer patients.Conclusion:The differential proteins,including RING finger protein 34,Wilms’tumor 1-associating protein,CD147 antigen,Tumor necrosis factor-inducible protein TSG-14 from plasmas group and Protein kinase NYD-SP9,Tumor necrosis factor receptor super family member 19 precursor,Histone-binding protein RBBP4,FADD protein,26S protease regulatory subunit p28,CD82 antigen,Autophagy protein 5 from sera group may be used as markers for HCC in blood.