The Role of Autonomous Wntless in Odontoblastic Differentiation of Mouse Dental Pulp Cells
Journal of Korean Dental Science
;
: 9-18, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-58138
ABSTRACT
PURPOSE:
Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin. MATERIALS ANDMETHODS:
To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction.RESULT:
Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout.CONCLUSION:
Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Dent
/
Facteurs de transcription
/
Adenoviridae
/
Pulpe dentaire
/
Dentine
/
Recombinases
/
Dentinogenèse
/
Allèles
/
Épithélium
/
Facteurs nucléaires-I
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Korean Dental Science
Année:
2016
Type:
Article
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