Study of Cryopreservation of Human Platelets / 中国输血杂志

ABSTRACT

Using the dimethylsulfoxide (DMSO)with final concentration of 4% as cryoproteclant, the human platelets were stored at - 30℃ and - 80℃ to provide cryopreserved platelets transfusion service. The results of in vivo and in vitro studies showed that DMSO could reversibly inhibit the platelet adhesion and its aggregation induced by the single aggregating agent. The platelet abhe-sion and aggregation could be recovered after washing and removing DMSO. The platelet aggregation caused by the combined aggregating agents wasn't influenced by DMSO. The adhesion, aggregation and resulting hypotonic shock reaction of cryopreserved platelets were used to be lower than those of fresh platelets, independent on the storage timed or 3 months). The quality of the platelets stored at - 80℃ was better than that at - 30℃. Being transfused with the platelets stored at - 80# could raise the platelet counts in patients, and effectively prevent and control the haemorrhage caused by thrombocytopenia.