Cloning and Screening of Mutant Human Hemoglobin Gene(?_(99) ,?_(82) Lys→Cys) / 中国输血杂志
Chinese Journal of Blood Transfusion
;
(12)1988.
Article
Dans Chinois
| WPRIM
| ID: wpr-582008
ABSTRACT
Objective:
To mutate the codons which code the lysine at loci ? 99 ,? 82 in human hemoglobin gene to the codons which code the cystein.Methods:
The objective gene to be mutated was cloned into pAlter Ex2 plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.Results:
The sequencing of clones obtained by preliminary screening showed that the anticipated results were reached without any unexpected mutation.Conclusion:
The results lay a foundation for the further development of research on expression and purification of recombinated mutant human hemoglobin gene.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Étude de dépistage
langue:
Chinois
Texte intégral:
Chinese Journal of Blood Transfusion
Année:
1988
Type:
Article
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