Adenovirue-mediated human p27kip1 gene transfection inhibits neointimal proliferation of rabbit carotid artery after balloon injury / 中国介入心脏病学杂志

ABSTRACT

Objective To study the effects of adenovirue-mediated p27kipl gene and its protein product overexpression on neointimal proliferation of rabbit carotid artery after balloon injury. Methods After rabbit arterial carotid injuried, injuried arterial segments were immediately infected by LacZ recombinant adenovirues (AdLacZ) and human p27kipl recombinant adenovirues (Adhp27kip1) in vivo, respectively. Western blot, x-gal stainning, HE stainning, immunochemistry and compute image system were used to analyze the expression of exogenous p27kipl gene and its protein product and the effects on neointimal proliferation. Results Comparison to AdLacZ infected or uninfected arterial segments, Adhp27kip1 infected segments overexpressed p27kipl protein, the peak of expression was in 7-14 d, expression lasted more than 4 weeks. After 4 weeks, in uninfected, AdLacZ infected or Adhp27kipl infected segments, the neointimal area was 1.106 mm2, 0.988 mm2 and 0.278 mm2, respecively; the rate of luminal stenosis was 87.07%, 65.40% and 32.14%, respectively. Conclusion Exogenous p27kipl gene and its protein product overexpression in injuried arterial segments could inhibit neointimal proliferation and luminal stenosis significantly after artery injury.