The role of apoptosis of ?-cells in the pathogenesis of Diabetis in OLETF, of obese type 2 diabetes rats / 中国糖尿病杂志

ABSTRACT

Objective To determine whether the ? cells apoptosis exits in the obesity associated type 2 diabetic rats OLETF and the relationship between the apoptosis and development of type 2 diabetes. Methods Twenty OLETFs and twenty LETOs as control were used. HE staining and TUNE were the way to examine apoptotic ? cell in pancreatic sections. Results (1) The incidence of diabetes in OLETF rats was 95%. None of diabetic rat was found among LETO rats. (2) Apoptotic cells only appeared in pancreatic sections from OLETF rats. (3) The percentage of islet ? cell apoptosis in OLETF rats was higher than that in LETO rats (P=1.26?10 -17 ). (4) In OLETF rats, the percentage of islet ? cells apoptosis was higher in the ages of 4 weeks after the onset of diabetes than that in the ages of four weeks before the onset of diabetes. Conclusion There was a high level of ? cell apoptosis in OLETF rats prior to the onset of diabetes and a further increase of apoptosis after diabetes.