Development of excitotoxicity model with primary hippocampal neurons and implications of viability essay / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-584219
ABSTRACT
Objective To study the injury and protective action of drugs on neurons, the model of glutamate excitotoxicity on primary cultured hippocampual neurons from new born rats were( set up. Methods)By use of trypan blue dye staning and testing the lactate dehydrogenase leakage from cultured neurons, to investigate the neuron survival rate. Results We found the injury of neurons was related with the concentration of glutamate. NMDAR non-competitive antagonist —MK-801 could protect the glutamate excitotoxic damage on neurons. Conclusion The glutamate results in neuron injury through NMDAR; the model of neuron culture was sufficient for glutamate-induced excitotoxicity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Laboratory Medicine
Année:
2003
Type:
Article
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