Resveratrol-induced apoptosis of Jurkat cell line in acute T lymphoblast leukemia / 吉林大学学报(医学版)

ABSTRACT

Objective To investigate the effect of resveratrol on proliferation inhibition,cell cycle arrest and apoptosis of Jurkat cell line in acute T lymphoblast leukemia.Methods MTT assay was used to determine the cell vitality.Wright-Giemsa,Hoechest 33258/PI staining and transmission electron microscope technique were used to detect the apoptosis status of Jurkat cells.The cell cycle arrest was analyzed by flow cytometry.(Results Resveratrol) had 64.01% inhibitory rate on the growth of Jurkat cells at 0.2 mmol?L~(-1) and inhibited the growth of Jurkat cells in dose-and time-dependent manner.24 h after treated with resveratrol,the typical features of apoptosis were observed under light and electron microscope in all treatment groups.Some nuclei showed bright blue under fluorescence microscope in the resveratrol-treated Jurkat cells,and the number of cells with bright blue fluorescence increased with time.Nuclei condensation and fragmentation were observed.Cell shrinkage,chromatin condensation,and marginalization were found by Wrigh-Giemsa staining and transmission electron microscope technique.By flow cytometry,62.57% of the cells were arrested at the S phase after exposured to(0.05 mmol?L~(-1)) resveratrol for 48 h,the rate of apoptotic cells to total cells was 12.01% in 0.05 mmol?L~(-1) treatment groups,and that in the control groups was 2.05%. Conclusion Resveratrol can inhibit the proliferation,cause S-phage arrest and induce the apoptosis of Jurkat cells.