Antifibrotic Effects of Phosphodiesterase (PDE) Inhibitor in Experimental Interstitial Fibrosis induced by Unilateral Ureteral Obstruction
Journal of the Korean Society of Pediatric Nephrology
;
: 85-91, 2002.
Article
Dans Coréen
| WPRIM
| ID: wpr-58636
ABSTRACT
PURPOSE:
Phosphodiesterase (PDE) inhibitor increases the cellular content of cAMP, and cAMP suppresses connective tissue growth factor (CTGF) expression induced by TGF-beta1. Therefore, we investigated whether PDE inhibitor suppresses renal fibrosis without suppression of TGF-beta. MATERIALS ANDMETHODS:
Renal interstitial fibrosis was produced by ligation of left ureter in Sprague-Dawley rats. Cilostazol, a selective PDE3 inhibitor, and dipyridamole, a hybrid PDE5, PDE6, and PDE8 inhibitor, were provided in drinking water for 7 days. In addition to the Masson-trichrome score of renal tissue, the concentration of fibronectin and TGF-beta1 in renal tissue-conditioned media was measured by ELISA.RESULTS:
Masson-trichrome score and fibronectin concentration were significantly lower in cilostazol-treated group compared to the control group (P<0.05). Though dipyridamole treatment seemed to suppress the Masson-trichrome score and fibronectin concentration too, the decrements were not statistically significant. There was no difference in TGF-beta1 concentration among the groups.CONCLUSION:
A selective PDE3 inhibitor cilostazol suppresses renal fibrosis without alteration of TGF-beta expression.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Uretère
/
Obstruction urétérale
/
Eau de boisson
/
Fibrose
/
Test ELISA
/
Facteur de croissance transformant bêta
/
Fibronectines
/
Rat Sprague-Dawley
/
Dipyridamole
/
Facteur de croissance transformant bêta-1
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of the Korean Society of Pediatric Nephrology
Année:
2002
Type:
Article
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