The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-alpha and IL-1beta Secretion / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 554-563, 2006.
Article
Dans Coréen
| WPRIM
| ID: wpr-58665
ABSTRACT
BACKGROUND:
PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear.METHODS:
This study examined the effects of PM exposure on the secretion of TNF-alpha and IL-1beta in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM (5~20 microgram/cm2), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted TNF-alpha and IL-beta in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the Lab-Tek(R) chamber slides were stained with immunocytochemical stains.RESULTS:
PM induced TNF-alpha and IL-1beta secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types.CONCLUSION:
PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of TNF-alpha and IL-1beta.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Asthme
/
Fibrose
/
Test ELISA
/
Technique de Western
/
Facteur de nécrose tumorale alpha
/
Macrophages alvéolaires
/
Bronchite chronique
/
Agents colorants
/
Maladies pulmonaires
/
Macrophages
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Tuberculosis and Respiratory Diseases
Année:
2006
Type:
Article
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