Effects of taurine on streptozotocin-induced apoptosis and expression of BCL-xL and BAX in rat pancreatic islet cells / 基础医学与临床

ABSTRACT

Objective To investigate the inhibition effect of taurine on the apoptosis of isolated pancreatic islet cells insulin secretion,the expression of BCL-xL and BAX induced by streptozotocin.Methods Isolated pancreatic islet cells from Wistar rats of 3 to 5 days were incubated in monolayer in vitro.(NO_2~-/NO_3~-) production,NOS activity,insulin secretion,expression of BCL-xL and BAX,apoptotic rate of islet cells after and DNA fragmentation in pancreatic islet cells after incubation with streptozotocin were measured,and the potential effects of taurine was investigated.Results Streptozotocin induced a significant increase in apoptotic rate of pancreatic islet cells(from(4.0?0.7)% to(40.5?5.7)%),DNA ladder appearance,a decrease of insulin content,up-regulation of the expression of BAX and down-regulation of the expression of BCL-xL(P