Study on the blood CD4~+CD25~+T cells of rats with experimental autoimmune encephalomyelitis / 临床神经病学杂志

ABSTRACT

Objective To study the number changes of blood CD4+CD25+T cells and its significance in rats with experimental autoimmune encephalomyelitis (EAE). Methods Wistar rats were induced by guinea pig spinal cord homogenate (GPSCH) to establish EAE models.The number of peripheral blood CD4+CD25+T cells in EAE was detected by flow cytometry and compared with normal rats.The behavior and the pathological changes of brain and spine cord of rats were observed to ascertain EAE model successful.Results The achievement ratio of EAE model was 48.9%.The number of peripheral blood CD4+CD25+T cells of EAE rats( 5.29?4.00) was significantly lower than that in the control group( 12.61?2.24, P