Changing characteristics of neural stem cells in the temporal lobe of cortex from human fetal brain at different age / 医学研究生学报

ABSTRACT

Objective:To investigate the changing characteristics of neural stem cells(NSC) in the temporal lobe of cortex from human fetal brain at different age.Methods:Ninety embryos delivered by induction labor with water bag at gestational age 16-36 w were collected,distribution,Shapes,growth modes and the number of NSC in the temporal lobe of cortex were determined with immunohistochemical method under light microscope. Results:NSC existed in the temporal lobe of cortex from human fetal brain and were round,elliptic or polygonal in shape,especially small round,NSC distributed mainly in cone cell and inner granule cell layers.NSC had enations from 0 to 2,nucleoli from 1 to 2 and rarefactive chromatin,nucli were large.Most NSC distributed in clones with a few NSC existed in a single mode among other nerve cells.There were no differences between groups in the distribution,shapes,species and growth modes of NSC in the temporal lobe of cortex,but the numbers of NSC gradually decreased with increase of age.Conclusion:There were no differences on the distribution,shapes,species and growth modes of NSCs in the temporal lobe of cortex from different gestational age,the number of NSC decreased with increase of age.