Relative Analysis of Pseudomonas aeruginosa Strain in Hospital Infection / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate the relationship of Pseudomonas aeruginosa strain in hospital infection. METHODS ?-Lactamase gene,aminoglycoside modification enzymes(AMEs)gene,and resistant antiseptic(qacE△1-sul1)gene were detected to mutidrug resistant P.aeruginosa with polymerase chain reaction(PCR) and we conducted cluster analysis of the molecule marked drug resistance gene. RESULTS PCR showed the positive ratios of TEM,CARB and VIM genes were 24.32%,43.24%,and 5.4%,respectively,While SHV,CTX-M,OXA-10 group,PER,GES,VEB,DHA,and IMP genes were all negative,the deleted ratio of oprD_2 gene was 86.49%;the positive ratios of aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰgenes were 8.11%,43.24%,18.91%,and 40.54%,respectively and the positive ratio of qacE△1 gene was 51.35%,There were clone transmitted phenomenon. CONCLUSIONS Hospital P.aeruginosa infection can induce clone transmitted hospital infection and it has fulminant prevalence.