Human lung carcinoma cell A549 express vascular endothelial grouth factor-C induced by hyaluronan / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-588461
ABSTRACT
Objective:
To investigate the expression of VEGF-C in human lung carcinoma cell A549 induced by hyaluronan(HA) in vitro.Methods:
A549 cells cultured in vitro were divided into three groups control group(C) cultured with free serum medium(FSM);HA treatment group 1(HA1) and group 2(HA2) cultured with FSM containing different concentration of HA(HA1 group 10?g/ml,HA2 group 20?g/ml).After 48 hours,VEGF-C expression were examined by reverse transcription-polymerase chain reaction(RT-PCR)? Western blot and immunofluorescence staining.Results:
Group C both VEGF-C mRNA and protein expression were weak positive.HA1 and HA2 groups both VEGF-C mRNA and protein expression were strong positive and increased significantly in a HA dose-depended way(P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Medical Postgraduates
Année:
2004
Type:
Article
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