Relationship between drug resistance of Candida albicans,gene expression associated with drug resistance and proteinase / 临床检验杂志

ABSTRACT

Objective To study the relationship between drug resistance and expression of the gene associated with drug resistance in Candida albicans,as well as the secretory proteinase activity of these isolates.Methods The minimum inhibitory concentrations(MIC)to fouconzole and itraconazole in 99 strains of Candida albicans isolated from respiratory tract were measured by broth microdilution method,the M27-A protocol recommended by NCCLS.The gene expressions of CDR1 and MDR1 were semi-quantitatively determined by RT-RCR.The secretory proteinase activity of these isolates was assayed by plate with medium containing bovine serum albumin.Results The rates of drug resistance to fluconzole and itraconazole were 4% and 12.1% respectively.CDR1 expression in the susceptible dose-dependent(S-DD)isolates was higher than those in the susceptible isolates and drug-resistant isolates(P