Constitution of Rat Glomerular Mesangial Cell Stably Expressing Decorin Gene / 复旦学报（医学版）

ABSTRACT

PurposeTo study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods Amplification of the rat decorin (DCN) eDNA by RT-PCR for constructing the plasmid pcDNA3.1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone.Results The recombinant eukaryotic expression plasmid, pcDNA3.1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. ConclusionsThese cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.