Preparation of Monoclonal Antibody Against "a" Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain / 生物化学与生物物理进展

ABSTRACT

Hepatitis B virus surface antigen with mutation on site 145 may cause omission in detection of hepatitis B virus with HBsAg diagnostic reagent. Monoclonal antibody against this mutation was obtained through hybridoma technique. The sub-class of the monoclonal antibody was determined to be IgG1(? type, the titer is 1∶9?104), and the monoclonal antibody has no cross-reaction to other hepatitis virus. The method of AC-ELISA which was established with monoclonal antibody and polyclonal antibody showed high analysing susceptibility for HBV mutant samples.