The Impact of Different Iodine Intakes on Type Ⅰ Iodothyronine Deiodinase Activity and mRNA Expression in Mouse Thyroid Tissue / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
; (12)2006.
Article
de Zh
| WPRIM
| ID: wpr-591139
Bibliothèque responsable:
WPRO
ABSTRACT
Thyroid function ultimately depends on appropriate iodine supply to the gland. Thyroid hormone deiodination is an intrinsic component of the thyroid hormone homeostasis. Type Ⅰ iodothyronine deiodinase (D1) plays an important role in thyroid hormone metabolism and has close relationship with thyroid function. Based on successfully establishing animal models of iodine deficiency and iodine excess in Babl/c mice (Babl/c mice were randomly divided into five groups: low iodine (LI), normal iodine (NI), five-fold iodine (5HI) , ten-fold iodine (10HI) and fifty-fold iodine (50HI) group. Three months and six months after admistration, they were sacrificed and thyroids were excised), the expression level of D1 mRNA were examined by using real time quantitative PCR method. D1 activity was analyzed by 125I-rT3 as substrate combined with ion-exchange chromatography. The thyroid hormone was measured with radioimmunoassay method. The data revealed that in the case of iodine deficiency, both D1 mRNA expression and D1 activity was greatly increased(compared with NI groups, P
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Progress in Biochemistry and Biophysics
Année:
2006
Type:
Article