Genotyping of ?-Lactamases and Preliminary Discovery of Acinetobacter-derived cephalosporinases AmpC Subtype from Acinetobacter baumannii / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate the genes associated ?-lactamases in Acinetobacter baumannii(ABA)isolated and preliminary discovery of Acinetobacter-derived cephalosporinases(ADC)-subtype in Shaoxing,Zhejiang.METHODS Eighteen ?-lactamases genes were detected by PCR,and a new ADC ?-lactamases gene fragments produced by PCR were sequenced and analyzed.RESULTS The positive rates of ADC-gene,TEM-gene and OXA-23 group-gene were 85.9%,27.1% and 58.8%,respectively.The other genes were not found in all 85 isolates tested.CONCLUSIONS There are very high positive percentages of ADC,TEM and OXA-23 group genes in A.baumannii isolated clinically and that is the important mechanism of multi-resistance.The ADC gene may be a novel subtype in lactamases(GenBank accession numberEF569590).