Hypoxia dependent HIF-1? upregulates the expression of HPA in human ovarian cancer cell line SKOV3 / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
Dans Zh
| WPRIM
| ID: wpr-591601
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To investigate the expression of hypoxia inducible factor-1?(HIF-1?) and heparanase(HPA),and their relationship during hypoxia in human ovarian cancer cell line SKOV3.Methods SKOV3 cells were incubated in different groups,immucytochemistry was applied to qualitatively assay HPA protein,Western-blot was used to detect the protein expression of HIF-1? and HPA,and mRNA expression was determined by RT-PCR.Results The positive expression of HPA localized in cytoplasma.Western-blot analysis revealed that the protein expression of HIF-1? was significantly increased(P
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Basic & Clinical Medicine
Année:
2006
Type:
Article