Hypoxia promotes proliferation of rabbit mesenchymal stem cells / 基础医学与临床

ABSTRACT

Objective To investigate the effect of transition between hypoxia and normoxia on proliferation of rabbit mesenchymal stem cells(rMSCs).Methods rMSCs expanded in hypoxia were divided into two groups:L-L group and L-N group.Moreover,rMSCs expanded in normoxia were also divided into two groups:N-N group and N-L group.The L-L and N-L groups were subsequently subcultured in hypoxia,and L-N and N-N groups in normoxia.Growth curve,colony-forming efficiency,glucose and lactate metabolism,and intracellular ROS generation of the four groups were examined.Results In L-L group cell density and colony-forming efficiency were the highest,and intracellular ROS generation was the lowest,whereas reversed results were obtained in N-N group.Cells cultured in hypoxic conditions exhibited higher glucose consumption,lactate production and yield coefficients of lactate from glucose compared to normoxic ones.Conclusion Hypoxia may enhance the proliferation of rMSCs in continuous subcultures.There is a potential link between intracellular ROS generation and expansion of rMSCs.