Hydrogen sulfide inhibits expression of intercellular adhesion molecule-1 in the pathogenesis of atherosclerosis in apoE knockout mice / 基础医学与临床

ABSTRACT

Objective To explore the effect of hydrogen sulfide(H2S) on intercellular adhesion molecule-1(ICAM-1) in the pathogenesis of atherosclerosis in apoE knockout(apoE-/-) mice.Methods Six week-old C57BL/6J and apoE-/-mice were divided into control C57BL/6J group,apoE-/-group,apoE-/-+ sodium hydrosulfide(NaHS,a donor of H2S) group and apoE-/-+ DL-propargylglycine(PPG,an inhibitor of H2S synthase).There were 8 mice in each group.They were fed chow diet for 10 weeks.Serum H2S was measured by sulfide electrode-based method,serum ICAM-1 was determined by ELISA.Aortic ICAM-1 mRNA was detected by RT-PCR.The changes of atherosclerosis plaque size were observed by oil red O staining.Results Serum H2Slevel significantly decreased in apoE-/-mice(P