Construction of humulus scandens pollen major allergen pcDNA3.1-LC2 DNA vaccine and study of its immunogenicity in mice / 西安交通大学学报(医学版)

ABSTRACT

Objective To construct humulus scandens pollen major allergen DNA vaccine pcDNA-LC2 and evaluate its immunogenicity. Methods Humulus scandens pollen allergen gene was digested from recombinant plasmid pTripIEx2-LC2 by EcoRⅠ and HindⅢ of restriction endonuclease, and then inserted into expression vector pcDNA3.1(-). A large amount of endotoxin pcDNA3.1-LC2 purified plasmid was extracted after successful construction, and animal experiment was carried out to study its immunocompetence. Results Sequencing results showed that 672bp in the coding sequence of frames of recombinant pcDNA3.1-LC2 was in line with the original base line, without deletion or mutation. pcDNA3.1-LC2 of pollen allergen humulus scandens allergen vaccinated BALB/c mice. Double immunodiffusion test showed that pcDNA3.1-LC2 could be expressed and produce antibodies in mice. Conclusion Humulus scandens grass pollen major allergen DNA vaccine pcDNA3.1-LC2 has been successfully constructed. It can be expressed in mice, produces humulus scandens grass pollen allergen specific antibody and has a good immunogenicity.